PandemieGrippaleV1, Main, Exploration, bibRecord, 003A40

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

Identifieur interne : 003A40 ( Main/Exploration ); précédent : 003A39; suivant : 003A41

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

Auteurs : Alexander Nagy [République tchèque] ; Veronika Vostinakova [République tchèque] ; Zuzana Pirchanova [République tchèque] ; Lenka Cernikova [République tchèque] ; Zuzana Dirbakova [Slovaquie] ; Miroslav Mojzis [Slovaquie] ; Helena Jirincova [République tchèque] ; Martina Havlickova [République tchèque] ; Adam Dan [Hongrie] ; Krisztina Ursu [Hongrie] ; Stefan Vilcek [Slovaquie] ; Jitka Hornickova [République tchèque]

Source :

Archives of virology [ 0304-8608 ] ; 2010.

RBID : Pascal:10-0265175

Descripteurs français

Pascal (Inist)
- Virus grippal A, Influenzavirus aviaire, Mammalia, Temps réel, Réaction chaîne polymérase RT, Détection.

English descriptors

KwdEn :
- Avian influenzavirus, Detection, Influenza A virus, Mammalia, Real time, Reverse transcription polymerase chain reaction.

Abstract

The objective of our study was to develop and evaluate a TaqMan real-time RT-PCR (RRT-PCR) assay for universal detection of influenza A (IA) viruses. The primers and LNA-modified octanucleotide probe were selected to correspond to extremely conserved regions of the membrane protein (MP) segment identified by a comprehensive bioinformatics analysis including 10,405 IA viruses MP sequences, i.e., all of the sequences of the Influenza Virus Sequence database collected as of August 20, 2009. The RRT-PCR has a detection limit of approximately five copies of target RNA/reaction and excellent reaction parameters tested in four IA viruses reference laboratories. The inclusivity of the assay was estimated at both the bioinformatic and the experimental level. Our results predicted that this RRT-PCR assay was able to detect 99.5% of known human IA virus strains, 99.84% of pandemic influenza A (H1N1) strains, 99.75% of avian strains, 98.89% of swine strains, 98.15% of equine strains, and 100% of influenza A viruses of other origin.

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Avian influenzavirus</term>
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<term>Reverse transcription polymerase chain reaction</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Virus grippal A</term>
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<front><div type="abstract" xml:lang="en">The objective of our study was to develop and evaluate a TaqMan real-time RT-PCR (RRT-PCR) assay for universal detection of influenza A (IA) viruses. The primers and LNA-modified octanucleotide probe were selected to correspond to extremely conserved regions of the membrane protein (MP) segment identified by a comprehensive bioinformatics analysis including 10,405 IA viruses MP sequences, i.e., all of the sequences of the Influenza Virus Sequence database collected as of August 20, 2009. The RRT-PCR has a detection limit of approximately five copies of target RNA/reaction and excellent reaction parameters tested in four IA viruses reference laboratories. The inclusivity of the assay was estimated at both the bioinformatic and the experimental level. Our results predicted that this RRT-PCR assay was able to detect 99.5% of known human IA virus strains, 99.84% of pandemic influenza A (H1N1) strains, 99.75% of avian strains, 98.89% of swine strains, 98.15% of equine strains, and 100% of influenza A viruses of other origin.</div>
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<affiliations><list><country><li>Hongrie</li>
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<li>Slovaquie</li>
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<name sortKey="Mojzis, Miroslav" sort="Mojzis, Miroslav" uniqKey="Mojzis M" first="Miroslav" last="Mojzis">Miroslav Mojzis</name>
<name sortKey="Vilcek, Stefan" sort="Vilcek, Stefan" uniqKey="Vilcek S" first="Stefan" last="Vilcek">Stefan Vilcek</name>
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<country name="Hongrie"><region name="Hongrie centrale"><name sortKey="Dan, Adam" sort="Dan, Adam" uniqKey="Dan A" first="Adam" last="Dan">Adam Dan</name>
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<name sortKey="Ursu, Krisztina" sort="Ursu, Krisztina" uniqKey="Ursu K" first="Krisztina" last="Ursu">Krisztina Ursu</name>
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   |texte=   Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species
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	Serveur d'exploration sur les pandémies grippales
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Serveur d'exploration sur les pandémies grippales

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

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